Hu, B. et al. Thermostable ionizable lipid-like nanoparticle (iLAND) for RNAi treatment of hyperlipidemia. Sci. Adv. 8, eabm1418 (2022).
Zhao, J. H. & Guo, H. S. RNA silencing: from discovery and elucidation to application and perspectives. J. Integr. Plant Biol. 64, 476–498 (2022).
Chen, Y. et al. Targeting Xkr8 via nanoparticle-mediated in situ co-delivery of siRNA and chemotherapy drugs for cancer immunochemotherapy. Nat. Nanotechnol. 18, 193–204 (2023).
Chen, X. et al. RNA interference-based therapy and its delivery systems. Cancer Metast. Rev. 37, 107–124 (2018).
Kanasty, R., Dorkin, J. R., Vegas, A. & Anderson, D. Delivery materials for siRNA therapeutics. Nat. Mater. 12, 967–977 (2013).
Kim, B., Park, J. H. & Sailor, M. J. Rekindling RNAi therapy: materials design requirements for in vivo siRNA delivery. Adv. Mater. 31, e1903637 (2019).
Jiang, X. et al. Oral delivery of nucleic acid therapeutics: challenges, strategies, and opportunities. Drug Discov. Today 28, 103507 (2023).
Liang, J. et al. Sphk2 RNAi nanoparticles suppress tumor growth via downregulating cancer cell derived exosomal microRNA. J. Control. Release 286, 348–357 (2018).
Zhou, X. et al. Tumour-derived extracellular vesicle membrane hybrid lipid nanovesicles enhance siRNA delivery by tumour-homing and intracellular freeway transportation. J. Extracell. Vesicles 11, e12198 (2022).
Zhuang, J. et al. Targeted gene silencing in vivo by platelet membrane-coated metal-organic framework nanoparticles. Sci. Adv. 6, eaaz6108 (2020).
Dammes, N. et al. Conformation-sensitive targeting of lipid nanoparticles for RNA therapeutics. Nat. Nanotechnol. 16, 1030–1038 (2021).
Blaby-Haas, C. E. & Merchant, S. S. Lysosome-related organelles as mediators of metal homeostasis. J. Biol. Chem. 289, 28129–28136 (2014).
Uddin, N., Binzel, D. W., Shu, D., Fu, T.-M. & Guo, P. Targeted delivery of RNAi to cancer cells using RNA-ligand displaying exosome. Acta. Pharm. Sin. B 13, 1383–1399 (2023).
Han, K. et al. A tumor targeted chimeric peptide for synergistic endosomal escape and therapy by dual-stage light manipulation. Adv. Funct. Mater. 25, 1248–1257 (2015).
Wittrup, A. et al. Visualizing lipid-formulated siRNA release from endosomes and target gene knockdown. Nat. Biotechnol. 33, 870–876 (2015).
Gilleron, J. et al. Image-based analysis of lipid nanoparticle-mediated siRNA delivery, intracellular trafficking and endosomal escape. Nat. Biotechnol. 31, 638–646 (2013).
Miller, J. B. & Siegwart, D. J. Design of synthetic materials for intracellular delivery of RNAs: from siRNA-mediated gene silencing to CRISPR/Cas gene editing. Nano Res. 11, 5310–5337 (2018).
Selby, L. I., Cortez-Jugo, C. M., Such, G. K. & Johnston, A. P. R. Nanoescapology: progress toward understanding the endosomal escape of polymeric nanoparticles. Wiley Interdiscip. Rev. Nanomed. Nanobiotechnol. 9, e1452 (2017).
Zhang, Y. et al. An antigen self-assembled and dendritic cell-targeted nanovaccine for enhanced immunity against cancer. Acta. Pharm. Sin. B 13, 3518–3534 (2023).
Kamerkar, S. et al. Exosomes facilitate therapeutic targeting of oncogenic KRAS in pancreatic cancer. Nature 546, 498–503 (2017).
Herrmann, I. K., Wood, M. J. A. & Fuhrmann, G. Extracellular vesicles as a next-generation drug delivery platform. Nat. Nanotechnol. 16, 748–759 (2021).
Kalluri, R. & LeBleu, V. S. The biology, function, and biomedical applications of exosomes. Science 367, eaau6977 (2020).
Mathieu, M., Martin-Jaular, L., Lavieu, G. & Théry, C. Specificities of secretion and uptake of exosomes and other extracellular vesicles for cell-to-cell communication. Nat. Cell Biol. 21, 9–17 (2019).
Mulcahy, L. A., Pink, R. C. & Carter, D. R. Routes and mechanisms of extracellular vesicle uptake. J. Extracell. Vesicles 3, 24641 (2014).
Tian, T. et al. Exosome uptake through clathrin-mediated endocytosis and macropinocytosis and mediating miR-21 delivery. J. Biol. Chem. 289, 22258–22267 (2014).
Cui, L. et al. Vesicle trafficking and vesicle fusion: mechanisms, biological functions, and their implications for potential disease therapy. Mol. Biomed. 3, 29 (2022).
Hindi, S. M. et al. Enveloped viruses pseudotyped with mammalian myogenic cell fusogens target skeletal muscle for gene delivery. Cell 186, 2062–2077.e17 (2023).
Ji, X. et al. In situ cell membrane fusion for engineered tumor cells by worm-like nanocell mimics. ACS Nano 14, 7462–7474 (2020).
Chen, P. et al. A plant-derived natural photosynthetic system for improving cell anabolism. Nature 612, 546–554 (2022).
Ho, N. T. et al. Membrane fusion and drug delivery with carbon nanotube porins. Proc. Natl Acad. Sci. USA 118, e2016974118 (2021).
Zheng, Z., Li, Z., Xu, C., Guo, B. & Guo, P. Folate-displaying exosome mediated cytosolic delivery of siRNA avoiding endosome trapping. J. Control. Release 311–312, 43–49 (2019).
Sanders, D. W. et al. SARS-CoV-2 requires cholesterol for viral entry and pathological syncytia formation. eLife 10, e65962 (2021).
Wang, C. et al. Different regions of synaptic vesicle membrane regulate VAMP2 conformation for the SNARE assembly. Nat. Commun. 11, 1531 (2020).
Nakato, M. et al. ABCA13 dysfunction associated with psychiatric disorders causes impaired cholesterol trafficking. J. Biol. Chem. 296, 100166 (2021).
Allen, J. A., Halverson-Tamboli, R. A. & Rasenick, M. M. Lipid raft microdomains and neurotransmitter signalling. Nat. Rev. Neurosci. 8, 128–140 (2007).
Linetti, A. et al. Cholesterol reduction impairs exocytosis of synaptic vesicles. J. Cell Sci. 123, 595–605 (2010).
Lötvall, J. et al. Minimal experimental requirements for definition of extracellular vesicles and their functions: a position statement from the International Society for Extracellular Vesicles. J. Extracell. Vesicles 3, 26913 (2014).
Düzgüneş, N. & Nir, S. Mechanisms and kinetics of liposome-cell interactions. Adv. Drug Deliv. Rev. 40, 3–18 (1999).
Kong, L., Askes, S. H. C., Bonnet, S., Kros, A. & Campbell, F. Temporal control of membrane fusion through photolabile PEGylation of liposome membranes. Angew. Chem. Int. Ed. 55, 1396–1400 (2016).
Skotland, T., Hessvik, N. P., Sandvig, K. & Llorente, A. Exosomal lipid composition and the role of ether lipids and phosphoinositides in exosome biology. J. Lipid Res. 60, 9–18 (2019).
Arnarez, C. et al. Dry Martini, a coarse-grained force field for lipid membrane simulations with implicit solvent. J. Chem. Theory Comput. 11, 260–275 (2015).
Samuel, M. et al. Oral administration of bovine milk-derived extracellular vesicles induces senescence in the primary tumor but accelerates cancer metastasis. Nat. Commun. 12, 3950 (2021).
Ziolkowski, W. et al. Methyl-beta-cyclodextrin induces mitochondrial cholesterol depletion and alters the mitochondrial structure and bioenergetics. FEBS Lett. 584, 4606–4610 (2010).
Chabanel, A. et al. Influence of cholesterol content on red cell membrane viscoelasticity and fluidity. Biophys. J. 44, 171–176 (1983).
Li, M. et al. Nanoscale imaging and mechanical analysis of Fc receptor-mediated macrophage phagocytosis against cancer cells. Langmuir 30, 1609–1621 (2014).
Zheng, D. W. et al. Hierarchical micro-/nanostructures from human hair for biomedical applications. Adv. Mater. 30, e1800836 (2018).
Qiu, Y. et al. Yolk-shell cationic liposomes overcome mucus and epithelial barriers for enhanced oral drug delivery. Giant 17, 100221 (2024).
Wang, X. et al. Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion. Nat. Biotechnol. 36, 946–949 (2018).
Zhao, E. et al. Spatial transcriptomics at subspot resolution with BayesSpace. Nat. Biotechnol. 39, 1375–1384 (2021).
Xue, C. et al. Programmably tiling rigidified DNA brick on gold nanoparticle as multi-functional shell for cancer-targeted delivery of siRNAs. Nat. Commun. 12, 2928 (2021).
Liu, H.-J. et al. Integrated combination treatment using a ‘smart’ chemotherapy and microRNA delivery system improves outcomes in an orthotopic colorectal cancer model. Adv. Funct. Mater. 28, 1801118 (2018).